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I would not mention what we just did, the CEO quietly advises as we sit on the tarmac in Seattle again, awaiting a customs officer to clear us to come home

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Research shows that nicotine in e-cigarettes increases the infiltration of inflammatory cells, including eosinophils, from the blood into the airways, stimulates the production of type 2 inflammatory cytokines such as IL-4, IL-5, and IL-13, and promotes mucin secretion, exacerbating airway inflammation and hyperresponsiveness, ultimately worsening asthma [66, 67]

However, the main drawback of this protocol is to have disparate flowrate (500 mL.s 1 ) compared to flowrate (1050 mL.s 1 ) used by vapers in real-life practice