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Primers were designed using ROCHE universal probe library primer design tool (version 2.45 and 2.48) and the sequences were as follow: p21 FW: 5- GAGGGCTAAGGCCGAAGATG -3, RV: 5- GCAGACCAGCCTGACAGATTTC -3 Rpl34 FW: 5- GTGAGGGACGAGGAGAGGTG -3, RV: 5- ACCATCCTGAGTGCCGCTTC -3 Slc7a11 FW: 5- TGGGTGGAACTGCTCGTAAT -3, RV: 5- AGGATGTAGCGTCCAAATGC -3 DDIT3 FW: 5- GCGACAGAGCCAGAATAACA -3, RV 5- GATGCACTTCCTTCTGGAACA -3 PTGS1 FV: 5- CCTCTTTCCAGGAGCTCACA -3, RV: 5- TCGATGTCACCGTACAGCTC -3 PYCR1 FW: 5- CTGACCATGTCAAGCCACTG -3, RV 5- TTCCCAGGGATAGCTGAGGT -3 NQO1 FW: 5- AGCGTTCGGTATTACGATCC -3, RV: 5- AGTACAATCAGGGCTCTTCTCG -3 ABCB1A FW: 5- GGGCATTTACTTCAAACTTGTCA -3, RV: 5- TTTACAAGCTTCATTTCCTAATTCAA -3 GCLC FW: 5- ATGATAGAACACGGGAGGAGAG -3, RV: 5- TGATCCTAAAGCGATTGTTCTTC -3 HMOX1 FW: 5- gtcaagcacagggtgacaga -3 RV 5- atcacctgcagctcctcaaa -3 For the qPCR reactions, 0.5 M primers were used1 l of Fast Sybr green gene expression master mix
Additionally, In vitro experiments have revealed that quercetin inhibits the polarization of M1 macrophages in RAW264.7 cells, resulting in reduced levels of TNF-, IL-1, IL-6, and NOS2, as well as decreased expression of Notch1
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